Category: Main Games osu! Stable


osu! builds from the Stable release stream.

Stable is the most recent version of osu! that is not on test phase. It currently renders through OpenGL 3.x, that allows Wine to get the same performance as Windows. It needs .NET Framework 4.0+ to work.

There are also the Beta and Cutting edge release streams that allow to test the latest development features. However they change frequently and older versions get obsolete quickly. Its compatibility level on Wine is usually the same as Stable.

Application Details:

Version: Stable
License: Free to use
Votes: 6
Latest Rating: Silver
Latest Wine Version Tested: 5.0.3

Maintainers: About Maintainership

Free Download osu!install.exe

Test Results

Old test results
The test results you have selected are very old and may not represent the current state of Wine.
Selected Test Results

What works

Everything except those that do not work.

What does not

  • Occasionally the keyboard will not respond in editor. Quit the editor and enter again might help. Else, restart osu!. 
  • The top menu of the editor is black, but will still respond to click and mouse hovers. 
  • Occasional stutters. But mostly won't. 
  • Might not be able to alt+tab out of the game if using a composited desktop environment. Still unsure about this. As a workaround tell Wine to emulate a virtual desktop for osu!. 
  • Song control (play/pause/next/etc) on main menu is missing, but song progress bar is there and it can respond to clicks.
  • Might have some audio delay. But offset is quite consistent so just tweak it from the universal offset option. 
  • Shaders. Results in white screen. 
  • Occasionally auto gain focus from other windows. Reason unknown.
  • Does not work with optirun -b virtualgl. But this is depreciated so don't care. 


What was not tested

  • Dragging MP3s to the osu! window to import them. I can't test this as I'm using it within an emulated virtual desktop by Wine. 
  • TaTaCon drum and Wiimote support, OS TabletPC support. Lack of hardware.
  • Exporting beatmaps from the editor. Should work anyway. 
  • osu!direct. But should work as downloading prebundled mapsets work. 
  • Social chatting apps integration.

Hardware tested


  • GPU:
  • Driver:

Additional Comments

selected in Test Results table below
Operating systemTest dateWine versionInstalls?Runs?Used
ShowUbuntu 20.04 "Focal" (+ variants like Kubuntu)Dec 31 20205.0.3Yes Yes YesSilverInvictus Tiberius 
ShowFreeBSD 12.2Dec 31 20205.0.3Yes No NoGarbageStreamNet 
ShowManjaro Linux 20.1Sep 19 20205.16Yes Yes YesGoldJames 
ShowantiX-19 x64-core "Marielle Franco"Sep 05 20205.16Yes No NoGarbageJakub Semeniuk 
ShowUbuntu 20.04 "Focal" (+ variants like Kubuntu)Aug 16 20205.15Yes Yes NoPlatinum13122002 

Known Bugs

Bug # Description Status Resolution Other apps affected
30941 osu! web-installer 20141028 fails with Wine-Mono: System.InvalidProgramException: Invalid IL code NEW View
44184 osu!: installer stuck in a loop NEW View
47066 Extremely low FPS in osu! on wine 4.6, unplayable on Wayland RESOLVED NOTOURBUG View
48507 osu! icons in the setting and music player are not being rendered UNCONFIRMED View
50111 osu! crashes since 20201110 version with wine-mono (needs native -> managed byref array marshalling) UNCONFIRMED View

Show all bugs

HowTo / Notes


Last update: 2020-12-31.

1) Get winetricks! (the easy way):

$ sudo wget '' -O /usr/local/bin/winetricks
$ sudo chmod +x /usr/local/bin/winetricks

2) Create a new 32-bit Wine prefix:

$ env WINEARCH=win32 WINEPREFIX=$HOME/.wineosu wineboot --init

If Wine prompts for installing Mono, click Cancel.

3) Install .NET Framework 4.0:

$ env WINEPREFIX=$HOME/.wineosu winetricks --force dotnet40

4) Download and install osu!:

$ mkdir 'osu!'
$ wget '!install.exe' --output-document='osu!/osu!.exe'
$ env PULSE_LATENCY_MSEC=40 WINEPREFIX=$HOME/.wineosu wine 'osu!/osu!.exe'
Running the game

Run in terminal:

$ env PULSE_LATENCY_MSEC=40 WINEPREFIX=$HOME/.wineosu wine 'osu!/osu!.exe'

Run in terminal (and detach from it so you can close the console window):

$ env PULSE_LATENCY_MSEC=40 WINEPREFIX=$HOME/.wineosu wine 'osu!/osu!.exe' &

Creating a shortcut: 

Create a new plain text file and copy the following:

[Desktop Entry]
Exec=env PULSE_LATENCY_MSEC=40 WINEPREFIX=$HOME/.wineosu wine ~/osu\\!/osu\\!.exe

Save this file as osu!.desktop in the folder ~/.local/share/applications and mark it as executable:

$ chmod +x '~/.local/share/applications/osu!.desktop'

Take the path to an osu! logo image and put it in the Icon field if you want to set an icon for the shortcut.


Last update: 2020-12-31.

Arch Linux specific:

  • osu! doesn't render anything on screen and exits with some X server error message: install lib32-libxcomposite package:

    $ sudo pacman -S lib32-libxcomposite
  • osu! can't connect to internet: install lib32-gnutls package:

    $ sudo pacman -S lib32-gnutls

Japanese characters no displaying correctly: install MS Meiryo font:

$ env WINEPREFIX=$HOME/.wineosu winetricks meiryo

Set language to Japanese to correctly display all kanjis.

This font doesn't include Korean glyphs.

Options menu symbols and audio controls are missing: install GDI+:

$ env WINEPREFIX=$HOME/.wineosu winetricks gdiplus

GDI+ installer is a ~538MB file. If your internet connection is slow or limited, consider instead taking a copy of gdiplus.dll from a Windows 7 installation and copying it to your Wine prefix, and then add manually a Native override for gdiplus.

Open winecfg:

$ env WINEPREFIX=$HOME/.wineosu winecfg

Go to Libraries tab, add gdiplus and set it to Native Then Builtin. Close all dialogs by clicking OK.

Some unusual image formats are not shown when using native GDI+: install windowscodecs through winetricks:

$ env WINEPREFIX=$HOME/.wineosu winetricks windowscodecs

Note: this workaround may break screenshots and interfere with other windows applications installed on the osu! wineprefix when trying to save files. If you need to disable it:

$ env WINEPREFIX=$HOME/.wineosu winecfg

Go to Libraries tab, find windowscodecs and set it to Builtin (Wine). Close all dialogs by clicking OK.

Audio latency, sound distortion or no audio at all: first try setting PULSE_LATENCY_MSEC environment variable to "40" before running osu!:

$ env PULSE_LATENCY_MSEC=40 WINEPREFIX=$HOME/.wineosu wine 'osu!/osu!.exe'

Other values could be tested in order to get lower latency or fix sound distortion/crackling.

Enabling "Audio compatibility mode" in the osu!'s options menu will switch to legacy DirectSound-based audio engine (osu! is now using WASAPI). It can solve some problems but has higher latency and some audio stuttering.

Also check out The Poon's guide and Franc[e]sco's guide.

Frame limiter is permanently locked at 60 fps:

Disable vertical sync by setting vblank_mode environment variable to "0 " before running osu!:

$ env vblank_mode=0 WINEPREFIX=$HOME/.wineosu wine 'osu!/osu!.exe'

This way osu! will not be able to enable VSync at runtime.

Video latency

Desktop environments usually draw on screen through a compositor than enable fancy window effects. This may cause lag that you can feel when moving the cursor.

Note: you will prefer to run osu! under since Wayland is not fully supported by most window managers.

  • GNOME 3 (Mutter): it should disable it automatically when running at borderless/fullscreen. If you're running on windowed mode or have many monitors, compositing may remain enabled unless osu! window fills all the screen output area.

  • KDE Plasma 5 (KWin): press Ctrl+Alt+F12 to disable/enable compositing.You can also add a window rule to block compositing when osu! starts and re-enable when it close.

  • Xfce 4 (Xfwm):

    Disable compositing:

    $ xfconf-query -c xfwm4 -p /general/use_compositing -s false

    Re-enable compositing:

    $ xfconf-query -c xfwm4 -p /general/use_compositing -s true

Other known problems:

  • My system is frozen!: if osu! freezes and you lose the control of your system, press Ctrl+Alt+F2 (to enter to a tty) login and enter the following to force closing osu!:
    $ pkill osu

    Press Ctrl+Alt+F1 (or Ctrl+Alt+F7 on some systems) to return to your desktop environment.

  • I ran osu but my cpu usage is at 100% and nothing happens: same as frozen system. Open a terminal and run:
    $ pkill osu

    Wait 10 seconds and try again.

  • Alt+tab doesn't work when Fullscreen is enabled: if you can't use Alt+Tab hotkey, disable Fullscreen mode inside osu!.

  • I can't switch to Windowed mode from Fullscreen/Borderless/Letterbox: close the game. Open osu!.«your_user_name».cfg in the osu! folder, and edit the next fields:
    Fullscreen = 0
    Letterboxing = 0
    Width = 800
    Height = 600

Please leave us a comment in Comments section below if you still have problems installing or running osu!.


The following comments are owned by whoever posted them. WineHQ is not responsible for what they say.

shortcut doesn't work and can't play without terminal open
by jack henderson on Tuesday December 22nd 2020, 8:12
when i try to put the exec into the terminal it works after removing some lines but no matter what it won't work through the .desktop and when i open up osu through the terminal if i close it osu doesn't stay opened
RE: shortcut doesn't work and can't play without terminal open
by Invictus Tiberius on Wednesday December 23rd 2020, 13:40
Add an ampersand & at the end of the command:
env PULSE_LATENCY_MSEC=40 WINEPREFIX=$HOME/.wineosu wine ~/osu\!/osu\!.exe &

This is a temporary solution if you don't manage to create the .desktop file and allows to close the terminal while osu! is running. Hope it helps!
Solved osu! can't display Chinese, Japenese or Korean glyphs even if you installed Ms Meiryo fonts
by Lys1257 on Sunday November 22nd 2020, 20:02
if you find that even install the MS Meiryo font can't help you to display CJK fonts, you shall install this

download this "UniFonts5.4" and install it, you shall only need to click next, if if appear a dialog with "??", just click it!AhC5R--MJQkvrkmkLF7pFM1wV6ZN?e=rJLTBp

env WINEPREFIX=$HOME/.wineosu winetricks ~/Downloads/UniFonts5.4.exe
RE: Solved osu! can't display Chinese, Japenese or Korean glyphs even if you installed Ms Meiryo fonts
by Lys1257 on Sunday November 22nd 2020, 20:06
Oh, replace the winetricks to wine

env WINEPREFIX=$HOME/.wineosu wine ~/Downloads/UniFonts5.4.exe
Workaround for beatmaps using black and white images while using native GDI+
by Nguyen Chinh Huu on Sunday November 22nd 2020, 1:44
Some beatmaps (such as use black and white images for the background/storyboard. If you are using native GDI+, they won't be shown. A workaround for it is installing `windowscodecs` from winetricks.
RE: Workaround for beatmaps using black and white images while using native GDI+
by Invictus Tiberius on Sunday November 22nd 2020, 6:45
Can confirm this happens and the workaround solves it. Thanks for the info!
by Lys1257 on Monday October 26th 2020, 7:53
if you are an Arch user, you might find when you are using Intel GPU, it works just fine.
but when you switch your GPU to NVIDIA, it seems like doesn't working after a quick flash of osu!
I find a solution, witch is pretty easy
try to install lib32-nvidia-utils

sudo pacman -S lib32-nvidia-utils
by Lys1257 on Monday October 26th 2020, 7:57
and by the way, you might find that after you installed this, steam can be open when you are using a NVIDIA GPU
Workaround for sound crackling or no sound with the new sound engine
by Nguyen Chinh Huu on Sunday January 5th 2020, 7:28
With the new osu!stable release with use WASAPI rather than the legacy DirectSound but have better (lower) latency, you might experiencing sound crackle or no sound. If that is the case, try setting the environment variable PULSE_LATENCY_MSEC=40 (or lower if your hardware handle that).
Or you can return to the old engine in Setting => Sound => Audio compatibility mode, which is not recommended because of the high latency and frequent shuttering.
RE: Workaround for sound crackling or no sound with the new sound engine
by Invictus Tiberius on Sunday January 5th 2020, 10:19
Tested on neon (ubuntu bionic), winehq-devel 5.0~rc4.

PULSE_LATENCY_MSEC=40, WASAPI: 67, 67, 50, 57, 63, 61, 52 (59.6ms)
PULSE_LATENCY_MSEC=40, Directsound: 104, 101, 107, 101, 105, 97 (102.5ms)
PULSE_LATENCY_MSEC=20, WASAPI: 30, 29, 22, 25, 24, 22 (25.3ms) --sound is distorted--
PULSE_LATENCY_MSEC=20, DirectSound: 101, 104, 99, 97, 101, 102 (100.6ms) --pretty much the same as before--

It's a nice workaround and applies to WineHQ packages. Adding to Troubleshooting section.
osu! will not run and results in a NET framework error
by Micah Jinkerson on Saturday December 14th 2019, 22:46
When running osu! with the provided command in the HowTo, Im given the following error:

I've tryed changing the WINE version to everything above XP but it still results in the same.
RE: osu! will not run and results in a NET framework error
by Micah Jinkerson on Saturday December 14th 2019, 22:55
Forgot to say im running xfce ubuntu 16.04 with WINE version 4.0.3
RE: osu! will not run and results in a NET framework error
by Invictus Tiberius on Thursday December 19th 2019, 4:05
Cannot replicate the bug. Just tested on Ubuntu 18.04 and Arch with wine-stable 4.0.3 and wine-staging 5.0-rc1 and it's working fine.
osu! shortcut not working
by Jason on Thursday September 12th 2019, 3:00
the command for making the shortcut will not work.
osu! folder in Application data in C drive not found
by Jason on Thursday September 12th 2019, 2:33
I have directed to C:\users\user\Local Settings\Application Data\ and i don't seem to find the osu! folder, did i miss a step?
RE: osu! folder in Application data in C drive not found
by Invictus Tiberius on Wednesday September 18th 2019, 11:22
By following these steps:

$ mkdir 'osu!'
$ wget '!install.exe' --output-document='osu!/osu!.exe'
$ env WINEPREFIX=$HOME/.wineosu wine 'osu!/osu!.exe'

A new folder called "osu!" is created directly in your personal user folder '/home/user/osu!'. Then, 'osu!install.exe' is renamed to 'osu!.exe'; that way it will install its files directly in '/home/user/osu!'.
If you want to install osu! in the usual way, try:

$ wget '!install.exe'
$ env WINEPREFIX=$HOME/.wineosu wine 'osu!install.exe'

Then, if you didn't choice another path, the osu! files will be found in '/home/user/.wineosu/drive_c/user/user/Local Settings/Application Data/osu!'
osu! show a black screen on Arch Linux
by Nguyen Chinh Huu on Monday August 19th 2019, 22:23
If osu! just show a black screen but there is music playing in the background or it doesn't run at all, and output an X error, try installing lib32-libxcomposite
RE: osu! show a black screen on Arch Linux
by Invictus Tiberius on Wednesday August 21st 2019, 21:11
Just tested on Arch and it works. Thanks for the info. Adding to Troubleshooting section.
Japanese Font
by Nguyen Chinh Huu on Monday April 29th 2019, 9:36
If you want to use GDI+ to fix the graphical glitches, you can still have osu! render Japanese using Meiryo fonts through winetricks: winetricks meiryo.
If you have installed cjkfonts or fakejapanese_vlgothic, you have to use --force flag to install it.
RE: Japanese Font
by Invictus Tiberius on Wednesday May 1st 2019, 4:08
Just tested and seems to work for most Japanese characters but not all. Anyway it's a better solution that doing nothing so I will add it to de guide. Thanks for the info.
RE: Japanese Font
by Nguyen Chinh Huu on Wednesday May 1st 2019, 10:19
Try changing the language of the game to Japanese (日本語) (the forth selectionn), and they will be displayed correctly. Maybe on other languages, the game thinks it is Chinese and try using Chinese font for them if there aren't any Japanese-only character.

by Gage Johnson on Friday January 4th 2019, 11:44
I get a 403 forbidden when winetricks tries to install dotnet462. Any help on a solution?
by Invictus Tiberius on Saturday January 5th 2019, 16:37
Try installing dotnet45; it's enough for osu!stable.
Gnome intellihide panels showing up
by André on Friday November 9th 2018, 11:23
On gnome desktop, sometimes (randomly) when i alt+tab the game, both panels (that were set to intellihide) were showing up if i touch the edge of the screen. To fix this just run the game on a virtual desktop with your fullscreen resolution.
Game is laggy and crashes.
by Robert on Thursday August 2nd 2018, 11:01
Everything was going well up until today when I wanted to play osu!, and it has a lot of lag spikes which make it unplayable. A few seconds into a map, or even barely loading the map, the game crashes.
RE: Game is laggy and crashes.
by Robert on Thursday August 2nd 2018, 11:22
Since I play osu! on a Seagate Expansion Drive, I thought of the fact that the USB was loose. So, I retightened it and it works perfectly! :)))))))))))))
New bug?
by Ena on Sunday June 3rd 2018, 12:21
I followed the instructions above and osu downloaded and installed properly, but on the first open it displays a message saying "osu was unable to obtain a graphics context. If this is the first time you've seen this please try restarting your PC. If this is a fresh install, you may be missing graphics drivers."
After you click ok, a window will pop up; its called "oh no!" and it will display the error (shown below):

#=z1U64elhN$6ScXutjLe68OMafNiDW: No available GL context found. ---> OpenTK.Graphics.GraphicsContextException: Failed to initialize EGL, error BAD_ALLOC.
at OpenTK.Platform.Egl.EglWindowInfo..ctor(IntPtr handle, IntPtr display, IntPtr surface)
at OpenTK.Platform.Egl.EglAnglePlatformFactory.CreateWindowInfo(IAngleWindowInfoInternal window_info, Int32 major, GraphicsContextFlags flags)
at OpenTK.Platform.Egl.EglAnglePlatformFactory.CreateGLContext(GraphicsMode mode, IWindowInfo window, IGraphicsContext shareContext, Boolean directRendering, Int32 major, Int32 minor, GraphicsContextFlags flags)
at OpenTK.Graphics.GraphicsContext..ctor(GraphicsMode mode, IWindowInfo window, IGraphicsContext shareContext, Int32 major, Int32 minor, GraphicsContextFlags flags)
at #=zN5FEz26lyeeReWfdrjnq99Map8xE.#=zegPSY_c=(Int32 #=zbrLuJSw=, Int32 #=zxzJ1FKg=, GraphicsContextFlags #=zzeNMliw=)
at #=zl1FmSAdVU5XcZFWz2wwT96w=.#=zh4G6cmQDc_G1(GraphicsContextFlags #=zzeNMliw=)
--- End of inner exception stack trace ---
at #=zl1FmSAdVU5XcZFWz2wwT96w=.#=zh4G6cmQDc_G1(GraphicsContextFlags #=zzeNMliw=)
at #=zl1FmSAdVU5XcZFWz2wwT96w=.#=zh4G6cmQDc_G1(GraphicsContextFlags #=zzeNMliw=)
at #=zl1FmSAdVU5XcZFWz2wwT96w=.#=zh4G6cmQDc_G1(GraphicsContextFlags #=zzeNMliw=)
at #=zl1FmSAdVU5XcZFWz2wwT96w=.OnHandleCreated(EventArgs #=z_Pvg3ZA=)
at #=zYTfEQdWXPSgMtNA4bKQNAjWhu_TZ.OnHandleCreated(EventArgs #=z_Pvg3ZA=)
at System.Windows.Forms.Control.WmCreate(Message& m)
at System.Windows.Forms.Control.WndProc(Message& m)
at System.Windows.Forms.ScrollableControl.WndProc(Message& m)
at System.Windows.Forms.ContainerControl.WndProc(Message& m)
at System.Windows.Forms.Form.WmCreate(Message& m)
at System.Windows.Forms.Form.WndProc(Message& m)
at #=zekbVG2TReeQyJ70gKx_9C2k=.WndProc(Message& #=z_sw5Hvg=)
at System.Windows.Forms.Control.ControlNativeWindow.OnMessage(Message& m)
at System.Windows.Forms.Control.ControlNativeWindow.WndProc(Message& m)
at System.Windows.Forms.NativeWindow.Callback(IntPtr hWnd, Int32 msg, IntPtr wparam, IntPtr lparam)

The update log looks like this:

6/3/2018 5:17:47 PM: Running repair (False)
6/3/2018 5:17:48 PM: Force update requested
6/3/2018 5:17:49 PM: CallbackStatus updated to Checking
6/3/2018 5:17:49 PM:
6/3/2018 5:17:49 PM: Beginning update thread
6/3/2018 5:17:49 PM: Stream: Stable40
6/3/2018 5:17:49 PM:
6/3/2018 5:17:49 PM: Running cleanup..
6/3/2018 5:17:49 PM: Cleanup successful!
6/3/2018 5:17:49 PM: Requesting update information...
6/3/2018 5:17:50 PM: discord-rpc.dll: CHANGED (cached)

I have version 3.0.1.
RE: New bug?
by Invictus Tiberius on Tuesday June 12th 2018, 8:44
Hi. This usually happens when there's no graphics hardware available. Verify you have correctly installed graphic drivers. I can't replicate this on my PC, so search for some info on google. You can also check Franc[e]sco's guide; he updated the guide some time ago and it is very complete.
Crashes when type korean characters in game
by Ian Snyder on Thursday May 3rd 2018, 1:05
osu! freezes when I type any Korean characters on osu! settings and beatmap search. However, It didn't freeze in bancho.
I'm using Ubuntu 18.04 LTS and Wine 3.0 from ubuntu repository.

Have any idea of this problem?
fixes graphical & font glitches
by osaka-takumi on Wednesday May 2nd 2018, 11:11
install Windows fonts on Linux
Please delete the internal font folder of wine
Otherwise font glitches will not be Fixed.
gdiplus override to Native, graphical glitches & CJK fonts will be fixed

by bbadadadadad bitch on Thursday April 26th 2018, 16:08
ssssorry nevermind got it aaa
cant get framework
by bbadadadadad bitch on Thursday April 26th 2018, 16:06
whenever i try to dowloaded it just fails on me, of course i open the dowloader on wine but since the machine i have doesnt work with it, cant seem to have it.
by osaka-takumi on Thursday February 22nd 2018, 5:44
osu!direct is very goood working
osu install problem
by Supashake YT on Wednesday February 21st 2018, 23:35
When I the osu installer is downloading osu, after the download countdown for what folder you want to put it in is done, it says 'An error occurred, please check you internet connection' even though my internet connection is fine

by Howard Zhang on Sunday January 21st 2018, 9:57
How do I launch it. Running osu!.exe doesn't work
RE: How to launch the game
by Invictus Tiberius on Sunday January 21st 2018, 11:42
Hi. osu! should create a shortcut when installing but if it is not your case, use the following command:

$ env WINEPREFIX=$HOME/.wineosu wine 'C:\«path_to_the_game»\osu!.exe'

Where "$HOME/.wineosu" is the absolute path to your wine-prefix and 'C:\«path_to_the_game»\" is a folder inside the wine-prefix where osu! is installed.

Be sure you followed all the guide to get it properly installed (I checked all steps for Wine 3.0-rc6 and it is working). Please leave us another comment if you are still in trouble.
RE: How to launch the game
by Howard Zhang on Monday January 22nd 2018, 20:16
Hey it did create a shortcut in the folder where osu! was installed. But when I click it, my mouse does the spiny thing and then it just stops.

And I didn't quite understand the command line. so do I put env WINEPREFIX=$HOME/.wineosu wine "c:\home/Umbryft/.wineosu/dosdevices/c:/users/Umbryft/Local Settings/Application Data/osu!\osu!.exe
RE: How to launch the game
by Howard Zhang on Monday January 22nd 2018, 20:22
I just checked task manager and it says it's running, but it doesn't pop up. But I should also tell you that after initial installation, osu ran fine. It was only after I closed it out it wouldn't pop up again. I would usually just use the env WINEPREFIX=$HOME/.wineosu wine 'osu!install.exe' command to get it up and running again but it stopped working so I don't know.

P.S. I forgot to add this in my last comment and I don't know how to edit it so I'm just adding It here I hope you don't mind hx.
RE: How to launch the game
by Invictus Tiberius on Wednesday January 24th 2018, 11:15
Hi, sorry for answering late. I updated the guide by adding an additional step explaining How to create a shortcut for osu! I hope it would be useful. If you can't still launch osu!, ask again. Anyways, I will try to explain some details here:

env WINEPREFIX=$HOME/.wineosu
Wine use this part to know what folder on your file system will be used as the "C:\" unit. Remember that Wine needs to "emulate" a Windows-like environment for your applications.

wine 'C:\Umbryft\Local Settings\Application Data\osu!\osu!.exe'
Inside the indicated "C:\" unit folder, Wine will execute the program that match to the indicated path, in this case, "osu!.exe". When using a terminal, you can put that path inside single quotes ' ', but in a ".desktop" shortcut, you need to use double quotes " " and append an additional inverted slash "\" to the existent:

env WINEPREFIX=$HOME/.wineosu wine "C:\\Users\\Umbryft\\Local Settings\\Application Data\\osu!\\osu!.exe"